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| Registros recuperados: 11 | |
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| Kincaid-smith, Julien; Boissier, Jerome; Allienne, Jean-francois; Oleaga, Ana; Djuikwo-teukeng, Felicite; Toulza, Eve. |
| For scientists working on gonochoric organisms, determining sex can be crucial for many biological questions and experimental studies, such as crossbreeding, but it can also be a challenging task, particularly when no sexual dimorphism is visible or cannot be directly observed. In metazoan parasites of the genus Schistosoma responsible for schistosomiasis, sex is genetically determined in the zygote with a female heterogametic ZW/ZZ system. Adult flukes have a pronounced sexual dimorphism, whereas the sexes of the larval stages are morphologically indistinguishable but can be distinguished uniquely by using molecular methods. Therefore, reliable methods are needed to identify the sex of larvae individuals. Here, we present an endpoint PCR-based assay using... |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00372/48333/71858.pdf |
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| Pinaud, Silvain; Portela, Julien; Duval, David; Nowacki, Fanny C.; Olive, Marie-aude; Allienne, Jean-francois; Galinier, Richard; Dheilly, Nolwenn M.; Kieffer-jaquinod, Sylvie; Mitta, Guillaume; Theron, Andre; Gourbal, Benjamin. |
| Discoveries made over the past ten years have provided evidence that invertebrate antiparasitic responses may be primed in a sustainable manner, leading to the failure of a secondary encounter with the same pathogen. This phenomenon called "immune priming" or "innate immune memory" was mainly phenomenological. The demonstration of this process remains to be obtained and the underlying mechanisms remain to be discovered and exhaustively tested with rigorous functional and molecular methods, to eliminate all alternative explanations. In order to achieve this ambitious aim, the present study focuses on the Lophotrochozoan snail, Biomphalaria glabrata, in which innate immune memory was recently reported. We provide herein the first evidence that a shift from a... |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00315/42664/71872.pdf |
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| Angora, Etienne K.; Allienne, Jean-francois; Rey, Olivier; Menan, Herve; Toure, Andre O.; Coulibaly, Jean T.; Raso, Giovanna; Yavo, William; N'Goran, Eliezer K.; Utzinger, Jurg; Balmer, Oliver; Boissier, Jerome. |
| Schistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Cote d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Cote d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman(R) FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A... |
| Tipo: Text |
Palavras-chave: Cote d'Ivoire; Hybrids; Molecular analysis; Schistosoma bovis; Schistosoma haematobium. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73604/73060.pdf |
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| Kincaid-smith, Julien; Mathieu-bégné, Eglantine; Chaparro, Cristian; Reguera-gomez, Marta; Mulero, Stephen; Allienne, Jean-francois; Toulza, Eve; Boissier, Jérôme; Lamberton, Poppy H. L.. |
| Species usually develop reproductive isolation mechanisms allowing them to avoid interbreeding. These preventive barriers can act before reproduction, “pre-zygotic barriers”, or after reproduction, “post-zygotic barriers”. Pre-zygotic barriers prevent unfavourable mating, while post-zygotic barriers determine the viability and selective success of the hybrid offspring. Hybridization in parasites and the underlying reproductive isolation mechanisms maintaining their genetic integrity have been overlooked. Using an integrated approach this work aims to quantify the relative importance of pre-zygotic barriers in Schistosoma haematobium x S. bovis crosses. These two co-endemic species cause schistosomiasis, one of the major debilitating parasitic diseases... |
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| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00693/80481/83621.pdf |
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| Ramalli, Lauriane; Mulero, Stephen; Noel, Harold; Chiappini, Jean-dominique; Vincent, Josselin; Barre-cardi, Helene; Malfait, Philippe; Normand, Guillaume; Busato, Florian; Gendrin, Vincent; Allienne, Jean-francois; Fillaux, Judith; Boissier, Jerome; Berry, Antoine. |
| Seven cases of urogenital schistosomiasis occurred in Corsica in 2015 and 2016. The episodes were related to exposure to the same river and involved the same parasite strain as an outbreak with 106 cases in summer 2013. The connection calls for further investigations on the presence of an animal reservoir and the survival of infested snails during winter. However, recontamination of the river from previously infected bathers remains the most likely hypothesis. Since 2015, seven cases of urogenital schistosomiasis reported exposure to the Cavu river in southern Corsica. They had no history of contact to fresh water in endemic areas. Here, we describe the cases indicating persistent schistosomal transmission linked to this river since 2013. To date, no... |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00615/72705/71744.pdf |
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| Djuikwo-teukeng, Felicite Flore; Simo, Alain Kouam; Allienne, Jean-francois; Rey, Olivier; Ngapagna, Arouna Njayou; Tchuem-tchuente, Louis Albert; Boissier, Jerome. |
| BackgroundSchistosomiasis is neglected tropical parasitic disease affecting both humans and animals. Due to the human health impact, population genetic studies have focused on the three main human-infecting schistosome species: Schistosoma mansoni, S. haematobium and S. japonicum. Here we present novel data on the population genetic structure of Schistosoma bovis, a highly widespread and prevalent schistosome infecting ruminants, and therefore of veterinary importance.MethodsAdult S. bovis were sampled in the two main abattoirs of Cameroon (Yaounde and Douala). Twenty-two cows originating from four distinct localities were sampled and a total of 218 parasites were recovered. All parasites were genotyped using a panel of 14 microsatellite markers and a... |
| Tipo: Text |
Palavras-chave: Schistosoma bovis; Barcoding; Population genetic structure. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00481/59267/62448.pdf |
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| Picard, Marion A. L.; Boissier, Jerome; Roquis, David; Grunau, Christoph; Allienne, Jean-francois; Duval, David; Toulza, Eve; Arancibia, Nathalie; Caffrey, Conor R.; Long, Thavy; Nidelet, Sabine; Rohmer, Marine; Cosseau, Celine. |
| Background Among more than 20,000 species of hermaphroditic trematodes, Schistosomatidae are unusual since they have evolved gonochorism. In schistosomes, sex is determined by a female heterogametic system, but phenotypic sexual dimorphism appears only after infection of the vertebrate definitive host. The completion of gonad maturation occurs even later, after pairing. To date, the molecular mechanisms that trigger the sexual differentiation in these species remain unknown, and in vivo studies on the developing schistosomulum stages are lacking. To study the molecular basis of sex determination and sexual differentiation in schistosomes, we investigated the whole transcriptome of the human parasite Schistosoma mansoni in a stage-and sex-comparative... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00358/46876/71864.pdf |
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| Portet, Anais; Pinaud, Silvain; Chaparro, Cristian; Galinier, Richard; Dheilly, Nolwenn M.; Portela, Julien; Charriere, Guillaume; Allienne, Jean-francois; Duval, David; Gourbal, Benjamin. |
| Selective pressures between hosts and their parasites can result in reciprocal evolution or adaptation of specific life history traits. Local adaptation of resident hosts and parasites should lead to increase parasite infectivity/virulence (higher compatibility) when infecting hosts from the same location (in sympatry) than from a foreign location (in allopatry). Analysis of geographic variations in compatibility phenotypes is the most common proxy used to infer local adaptation. However, in some cases, allopatric host-parasite systems demonstrate similar or greater compatibility than in sympatry. In such cases, the potential for local adaptation remains unclear. Here, we study the interaction between Schistosoma and its vector snail Biomphalaria in which... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00488/59982/63214.pdf |
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| Grech-angelini, Sebastien; Stachurski, Frederic; Lancelot, Renaud; Boissier, Jerome; Allienne, Jean-francois; Marco, Sylvain; Maestrini, Oscar; Uilenberg, Gerrit. |
| Background: Corsica is a mountainous French island in the north-western Mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. Despite a favourable context for ticks and tick-borne diseases (TBDs), the tick fauna of Corsica has not systematically been investigated. Methods: For one year (May 2014-May 2015), a survey of ticks infesting cattle was performed in the three Corsican cattle slaughterhouses. Two of these were visited monthly and one quarterly; the ticks were manually collected, just after flaying. Ticks were identified on their morphology; when necessary, some specimens were also molecularly identified by sequencing mitochondrial cox1 (cytochrome c oxidase subunit... |
| Tipo: Text |
Palavras-chave: Ticks (Ixodidae); Cattle; Domestic animals; Wild animals; Molecular identification; Corsica. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00361/47191/71863.pdf |
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| Registros recuperados: 11 | |
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